Division of Biophotonics and Imaging

FacultyServices Projects PublicationsResearch Staff

Main objective of the division is to take up and ensure high standard research in the field of nanomaterials for Bionanophotonics. The lab’s main areas of research themes include development and application of biomaterials, mainly bionanomaterials in the field of MR, CT and optical imaging and photodynamic and photothermal therapy. It is a comparatively new lab which also mentors the need of all laser based activities of the institute, both therapeutic and diagnostic and other biomedical application. We also work in the use of Spectroscopic techniques and spectral mapping and imaging for the early diagnosis of various diseases and the classification and discrimination of different pathologies using these techniques. These works focus on the hypothesis that the concept of optical pathology will be practical in the near future.

  1. He-Ne Laser for experimental purpose
  2. 810 nm diode laser for experimental purpose
  3. Spectrofluorometer (Fluorolog III, Jobin Yvon USA) : It is a double monochromator instrument capable of measuring the fluorescence spectrum as a function of an excitation wavelength. Solid and liquid samples and samples which could only be accessed remotely can also be studied using this equipment. It is suitable for measuring fluorescence emission spectrum from highl fluorescing samples like tissue and blood.
  4. Xenogen IVIS Spectrum - Optical Imaging system : The IVIS Spectrum is the newest and one of the most versatile and advanced in vivo imaging system. It facilitates non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. It can be used for in vivo imaging using bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also can be used to acquire 3D tomography for both fluorescent and bioluminescent reporters. In addition, imaging of multiple fluorescent reporters can also be performed using the spectral unmixing facility.

The department of Biophotonics and Imaging is mainly involved in the development of nanomaterials for biomedical applications mainly in the field of sensing, therapy and imaging. We have already designed a gold quantum cluster based biosensor for the detection of urea from whole blood. We have also developed several systems which can be used as MRI contrast agents. This includes both T1 and T2 contrast agents. Fluorescence spectroscopic techniques developed in the lab very effectively differentiates normal and diseased tissues with very high selectivity and sensitivity. The technique has been demonstrated successfully in the brain tumor tissue, oral tissue and in the clinical set up of oral cavity disorders. Other ongoing work of the lab are quantum dot-CNT system for imaging and therapy, zinc detection using nanoprobes, iron oxide based MRI contrast agents, Gold nanorods for PDT etc. Other research work based on various applications of lasers in medicine including standardisation of laser based therapeutic and diagnostic procedures for clinical applications is also going on. The department is also engaged in the development of low cost diagnostic systems using fluorescence and diffuse reflectance spectra and imaging. In vivo and ex vivo Fluorescence Spectroscopy, Imaging and Image processing also form part of our work.

Ongoing Projects
  1. "Development of Iron oxide nanoparticles for organ specific molecular imaging" funded by BRNS, DAE, Govt. of India
  2. "Quantum dot conjugated single walled carbon nanotubes for imaging and therapy" funded by DST, Govt. of India
  3. "Detection of Zinc using ratiometric fluorescent molecular probes" funded by DBT, Govt. of India
  4. "Gold Nanorods for targeted photodynamic therapy and fluorescence imaging" funded by ICMR, Govt. of India
  1. S. Jiji, K. A.Smitha, A.K Gupta, V.P. M.Pillai1, R.S Jayasree, Segmentation and volumetric analysis of the caudate nucleus in Alzheimer's Disease (Accepted in European Journal of radiology)
  2. Shaiju. S Nazeer, Ariya Saraswathy , A.K Gupta, R.S. Jayasree,Fluorescence spectroscopy as a highly potential single entity tool to identify chromophores and fluorophores: a study on neoplastic human brain lesions (Accepted in Journal of Biomedical Optics)
  3. M. Nidhin , Shaiju S Nazeer , R.S Jayasree , M. S. Kiran , B. U Nair and K.J Sreeram, Flower Shaped Assembly of Cobalt Ferrite Nanoparticles:Application as T2 Contrast Agent in MRI, RSC Advances RSC Adv., 2013, 3, 6906–6912 (DOI:10.1039/C3RA23232H)
  4. Lakshmi V. Nair, Divya S. Philips, R.S.Jayasree, A. Ajayaghosh A Near-Infrared Fluorescent Nanosensor (AuC@Urease) for the Selective Detection of Blood Urea, Small (DOI: 10.1002/smll.201300213)
  5. K A. Smitha, A.K Gupta , R. S. Jayasree, Total magnitude of diffusion tensor imaging as an effective tool for the differentiation of glioma, European Journal of Radiology 82(5):857-6, 2013 (doi.org/10.1016/j.ejrad.2012.12.027,) (Featured in MDLinx site which is world's most current index of articles that matter in the daily lives of physicians and other healthcare professionals)
  6. R.S. Jayasree, V.B. Sheshanath, V.K. Sumy, D. McNaughton, S.J. Langford Photodynamic effect of novel octaphosphante porphyrin evaluated by Raman spectroscopy and fluorescence microscopy, Photodiagnosis and Photodynamic Therapy, 8, 192, 2011.
  7. Nazeer Shaiju S, Ariya S, Asish R, Salim Haris P, Anita B, Arun Kumar G, Jayasree RS. Habits with killer instincts: in vivo analysis on the severity of oral mucosal alterations using autofluorescence spectroscopy. J Biomed Opt., 16, 087006, 2011.
  8. Haris PS, Balan A, Jayasree RS, Gupta AK. Autofluorescence spectroscopy for the in vivo evaluation of oral submucous fibrosis. Photomed Laser Surg., 27, 757-61, 2009.
  9. Jayasree RS, Gupta AK, Bodhey NK, Mohanty M. Effect of 980-nm diode laser and 1064-nm Nd:YAG laser on the intervertebral disc--in vitro and in vivo studies. Photomed Laser Surg., 27, 547-52, 2009. (Featured by NewsRx as Hot topics in October 10th, 2009)
  10. Saraswathy A, Jayasree RS, Baiju KV, Gupta AK, Pillai VP. Optimum wavelength for the differentiation of brain tumor tissue using autofluorescence spectroscopy. Photomed Laser Surg., 27, 425-33, 2009.