Pathology

FacultyServices Projects PublicationsResearch Staff

Services
  1. HISTOPATHOLOGY (Biopsy Request Form)

    The histopathology laboratory is well-equipped with various histochemical stains and immunohistochemistry for tissue diagnosis pertaining to the disciplines of neurosciences and cardiothoracic surgery.

    1. Intraoperative tissue diagnosis
    2. Squash smear preparation and/or cryosections
      Sample: Fresh tissue in a clean container

    3. Immunohistochemistry
    4. Immunohistochemistry is performed by manual staining method using standardized procedure.
      Sample: Paraffin blocks or tissue in formalin
      The list of available antibodies are:

      EntityAntibodies
      GliomaRoutine markers: GFAP, OLIG2, ATRX, p53
      Mutant antibodies: R132H-mutant IDH1
      K27M-mutant H3 (Diffuse midline glioma)
      G34R-mutant H3.3
      Others: L1CAM (Ependymoma)
      CNS embryonal tumorRoutine marker: Synaptophysin
      Medulloblastoma: Beta-catenin, YAP1
      Atypical teratoid/rhabdoid tumor (AT/RT): INI1 (SMARCB1)
      Embryonal tumor with multilayered rosettes (ETMR): LIN28A
      Pituitary adenomaHormones: GH, Prolactin, ACTH, TSH-β, FSH-β, LH-α, LH-β
      Transcription factors: TPIT, PIT1, GATA2
      Others: CAM5.2, p53, MIB1
      Meningioma
      Solitary fibrous tumor/ Haemangiopericytoma
      Routine marker: EMA, CD34
      Specific marker for SFT/HPC: STAT6
      Epilepsy surgery sample (Hippocampal sclerosis, focal cortical dysplasia, tumours, etc.) NeuN
      phosphorylated neurofilament (pNF),
      non- phosphorylated neurofilament (NF)
      GFAP
      CD34 (Ganglioglioma)
      ImmunoglobulinIgG, IgG4
      Germ cell tumorGerminoma: OCT4, CD117, PLAP
      Embryonal carcinoma: OCT4, CD30
      Yolk sac tumor: Alpha-fetoprotein
      Lung carcinomaSquamous cell carcinoma: CK5/6, p63, p40
      Adenocarcinoma: TTF1, Napsin A, CK7, CK20
      Thymomap63, TdT, PAX8, CD3
      Neuroendocrine tumorsSynaptophysin, Chromogranin, CD56
      Other basic markersMIB-1
      PanCK, CK7, CK20, CAM5.2, EMA
      S-100, GFAP, OLIG2
      Vimentin, Desmin, Myogenin, SMA, CD34
      LCA, CD20, CD3, CD5, CD99, CD138

      Charges

      Biopsy tissue specimenRs. 1000/-
      Paraffin blocksRs. 500/-
      Immunohistochemistry-1 markerRs. 1000/-
      Immunohistochemistry-2 markersRs. 2000/-
      NImmunohistochemistry-3 or more markersRs. 3000/-
      Immunohistochemistry-7 or more markersRs. 4000/-
      Immunohistochemistry-epilepsy samplesRs. 5000/-
      Immunohistochemistry- R132H-mutant IDH1Rs. 1000/-
      Immunohistochemistry-INI1Rs. 500/-
    5. Muscle, nerve and skin biopsies
    6. Sample: Muscle (for enzyme histochemistry and immunohistochemistry)

      • Fresh muscle wrapped in saline moistened gauze to reach the lab within 30 minutes
      • Samples will be received between 8:30 a.m. and 1:30 p.m.
      • The laboratory should be informed 1 day in advance before sending samples

      Nerve: 2.5% glutaraldehyde

      Skin: 2.5% glutaraldehyde

      The tests available are:

    Fresh Muscle biopsyEnzyme histochemistryModified Gomori's trichrome (MGT)
    Nicotinamide adenine dinucleotide-tetrazolium reductase (NADH-TR)
    Succinate dehydrogenase (SDH)
    Cytochrome oxidase (COX-SDH)
    ATPase at pH 9.5
    ImmunohistochemistryFast and slow myosin
    Dystrophin- rod domain, N-terminal, C-terminal
    α, β, γ, δ- Sarcoglycans
    Merosin
    Emerin
    Dysferlin
    OthersPeriodic acid-Schiff (PAS)
    Oil red O
    Nerve biopsy Kulchitsky Pal (K-Pal) for myelin
    Cresyl violet for metachromatic granules
    Skin biopsyFor Lafora bodiesLugol's iodine
    PAS-D

    Charges

    Muscle enzyme histochemistryRs. 2500/-
    Muscle immunohistochemistry- Dystrophin panel (rod domain, N-terminal, C-terminal and Merosin)Rs. 1000/-
    Muscle immunohistochemistry- Sarcoglycan panel (α, β, γ, δ- Sarcoglycans, Dysferlin and Merosin)Rs. 1500/-
    Muscle immunohistochemistry- Single antibodyRs. 500/-
    Nerve biopsyRs. 1500/-
    Skin biopsyRs. 1000/-
  2. CYTOLOGY (Request Form)
    1. CSF and body fluids for morphology/malignant cells
    2. Sample:

      • Any body fluid in clean container
      • Samples should be sent soon after collection
      • In case of any delay, samples should be stored in refrigerator 2-8ºC (Do not freeze)
    3. Fine needle aspiration cytology (FNAC)
  3. IMMUNOLOGY (Request Form)
    TestsSampleCost
    ELISA-based tests
    Anti-neutrophil cytoplasmic antibody-IgG (pANCA and c-ANCA)SerumRs. 400/-
    Antiphospholipid antibody (APLA)- IgMSerumRs. 350/-
    Antiphospholipid antibody (APLA)- IgGSerumRs. 350/-
    Anti-acetylcholine receptor antibody IgGSerumRs. 1750/-
    Anti-MuSK antibody IgGSerumRs. 520/-
    Anti-titin antibodySerumRs. 350/-
    Anti-thyroglobulin (TG) IgGSerumRs. 350/-
    Anti-thyroid peroxidase (TPO) antibody IgGSerumRs. 460/-
    Immunoblot tests
    ANA profile with Mi2 and Ku (Mi-2, Ku, nRNP/Sm, Sm, SS-A, Ro-52, SS-B, Scl-70, PM-Scl100, Jo-1, CENP-B, PCNA, dsDNA, Nucleosome, Histone, Rib P-protein, AMA-M2)SerumRs. 2000/-
    Paraneoplastic Neurologic profile (Neuronal antigen profile 2) (Amphiphysin, CV2, PNMA2(Ma2/Ta), Ri, Yo, Hu, Recoverin, SOX1, Titin, ZIC4, GAD65, Tr (DNER)SerumRs. 4500/-
    Anti-Ganglioside profile (GM1, GM2, GM3, GD1a, GD1b, GT1b, GQ1b)SerumRs. 2000/-
    Myositis Antigens IgG Profile (Mi-2, Ku, PM-Scl100, PM-Scl75, Jo-1, SRP, PL-7, PL-12, EJ, OJ, Ro-52)SerumRs. 2800/-
    Indirect Immunofluorescence-based tests
    Antinuclear antibody (ANA)SerumRs. 400/-
    Anti-ds DNASerumRs. 350/-
    Anti-VGKC-Associated Proteins (LGI1 & CASPR2)
    CSF
    Serum
    Serum and/or
    CSF

    Rs. 4000/-
    Rs. 4000/-
    Anti-Glutamate receptor (type NMDA)
    CSF
    Serum
    Serum and/or
    CSF

    Rs. 3500/-
    Rs. 3500/-
    Antibodies against Aquaporin4 and MOGSerum and/or CSFRs. 3000/-
    Autoimmune encephalitis panel (detects antibodies against LGI1, CASPR2, AMPAR1, AMPAR2, NMDAR, GABARB1/B2)Serum and/or CSFRs. 11000/-
    Oligoclonal band assay by Isoelectric Focusing and immunoblotting of CSF and serumCSF and serum (Paired sample collected around the same time)Rs. 5500/-

    Samples:

    • Serum samples: 2 ml blood to be sent in vacutainer without any anticoagulant (red capped)
    • CSF samples: 1-2 ml in a clean and plain vial
    • Samples should reach the laboratory as soon as possible after collection
    • Samples will be received between 8:30 a.m. and 3:30 p.m.
  4. Autopsy
    • Autopsies will be performed whenever required.
    • Informed consent from the next of kin for the autopsy (including the extent of autopsy) should be obtained.
    • Faculty should be informed after the consent is obtained.
    • For autopsies after working hours the faculty can be contacted through the reception.

For any additional details to contact 0471-2524594, 494, 574, 605, 195

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